Supplies required for sequencing

Description Recommended source Alternative source Note
ABI Big Dye Terminator (BDT) v3.1 JBPC ABI Only v3.1 please (keep cold)
MicroAmp Optical 96-Well Reaction Plate for cyclesequencing MBL stockroom* ABI, Corning Thermowell Gold -
Costar Thermowell Plate Tape MBL stockroom Fisher Scientific
any adhesive plate sealer will do
5X Reaction Buffer (for cyclesequencing w/BDT) make your own** ABI store at 4ºC
Hi-Di Formamide with EDTA, 25 ml/bottle ABI None -
Isopropanol MBL stockroom Fisher Scientific
DMSO MBL stockroom Fisher Scientific
Paper towels - - -
Tips for multi-channel pipettor - - -
* The MBL stockroom is located in the basement of the Lillie building and accepts WHOI charge numbers and some credit cards.
** 5 X Rxn Buffer = 400mM Tris-HCl, pH9.0 containing 10mM MgCl2 (keep refrigerated).

Miscellaneous part numbers and sources for reference:

Vendor Part # Description Note
ABI 4337454 ABI Big Dye Terminator (BDT) v3.1 Cycle Sequencing Ready Reaction Kit, 24 rxns larger kits available
ABI 4336697 5X Reaction Buffer, 1ml bottle
ABI 4311320 Hi-Di Formamide with EDTA, 25 ml bottle
ABI N801-0560 MicroAmp Optical 96-Well Reaction Plate, 10 plates
ABI N9302563 Cover Mat for 96 Well Optical Plate, 5/box (reusable)
ABI 4305505 MicroAmp Optical 384-Well Reaction Plate, 50 plates for 384's
Robbins Scientific / APOGENT
1044-39-6 Robbins CycleSeal 384 Well Plate Sealer, 20 sheets
(These are the only 384 plate sealers that work for us)
for 384's
Fisher Scientific
07-200-684 Costar Thermowell Plate Tape, 100/case Costar part #6570
SP010-1 M13 Universal Primer, 30 µg (forward) [one of either primer per run] example
SP030-1 M13 Reverse Primer, 30 µg (reverse) [one of either primer per run] example

Last updated 11/05/06