Description
Recommended source
Alternative source
Note
ABI Big Dye Terminator (BDT) v3.1
JBPC
ABI
Only v3.1 please (keep cold)
MicroAmp Optical 96-Well Reaction Plate for cyclesequencing
MBL stockroom*
ABI, Corning Thermowell Gold
-
Costar Thermowell Plate Tape
MBL stockroom
Fisher Scientific
(800-766-7000)
any adhesive plate sealer will do
5X Reaction Buffer (for cyclesequencing w/BDT)
make your own**
ABI
store at 4ºC
Hi-Di Formamide with EDTA, 25 ml/bottle
ABI
None
-
Isopropanol
MBL stockroom
Fisher Scientific
(800-766-7000)
-
DMSO
MBL stockroom
Fisher Scientific
(800-766-7000)
-
Paper towels
-
-
-
Tips for multi-channel pipettor
-
-
-
* The MBL stockroom is located in the basement of the Lillie building and accepts WHOI charge numbers and some credit cards.
** 5 X Rxn Buffer = 400mM Tris-HCl, pH9.0 containing 10mM MgCl2 (keep refrigerated).
Miscellaneous part numbers and sources for reference:
Vendor | Part # | Description | Note |
ABI | 4337454 | ABI Big Dye Terminator (BDT) v3.1 Cycle Sequencing Ready Reaction Kit, 24 rxns | larger kits available |
ABI | 4336697 | 5X Reaction Buffer, 1ml bottle | |
ABI | 4311320 | Hi-Di Formamide with EDTA, 25 ml bottle | |
ABI | N801-0560 | MicroAmp Optical 96-Well Reaction Plate, 10 plates | |
ABI | N9302563 | Cover Mat for 96 Well Optical Plate, 5/box (reusable) | |
ABI | 4305505 | MicroAmp Optical 384-Well Reaction Plate, 50 plates | for 384's |
Robbins Scientific / APOGENT (800-752-8585) |
1044-39-6 | Robbins CycleSeal 384 Well Plate Sealer, 20 sheets (These are the only 384 plate sealers that work for us) |
for 384's |
Fisher Scientific (800-766-7000) |
07-200-684 | Costar Thermowell Plate Tape, 100/case | Costar part #6570 |
Operon (800-688-2248) |
SP010-1 | M13 Universal Primer, 30 µg (forward) [one of either primer per run] | example |
Operon (800-688-2248) |
SP030-1 | M13 Reverse Primer, 30 µg (reverse) [one of either primer per run] | example |
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